Experimental parametersExperimental parametersis expensive wine, Can we get a way to tell analytically if there was a difference between top shelf whiskey and lower quality whiskey. So Gas chromatography MS is the fastest way to analysis. Of course, 2ml autosampler vial is needed in this experiment.
2ml HPLC vial Experimental
As the lucidity, the experimenter looked for 5 different whiskeys to test. The small sample of each of whiskeys injected into 2ml HPLC vials. Because the main element of whiskey are ethanol and water, the experiment is not too difficult. The experiment used MXT-Wax Column.
When whiskey samples injected into 2ml HPLC vial, the Gas chromatography instrument began to work. The experimenter do run the experiment in standard. But he found serverl studies that showed the composition of the sample and the chromatogram match almost exactly in retention times.
Whiskey Experimental parameters
| Lucidity MiniGC Conditions |
|
| Carrier Gas |
Helium |
| Control |
Pressure |
| Flow |
1mL/min |
| Split |
10:1 |
| Column |
30 m x 0.25 mm, 0.25 μm |
| Injector |
250 °C |
| FID |
300 °C |
Results
Analysis from the
2ml HPLC vial sample, There were 8 main peaks for each of the whiskeys. They were as follows:
1. Ethyl acetate
2. Methanol
3. Ethanol
4. 1-Propanol
5. Isobutyl alcohol
6. 3-Methyl-1-butanol
7. Acetic acid
8. Phenyl ethyl alcohol
Each of these elements are responsible for some of the distinctive taste and smells of these whiskeys. Each of these whiskeys had a different concentration of these compounds. The lower priced whiskeys had low concentrations of the ethyl acetate, isobutyl alcohol and phenyl ethyl alcohol and higher concentrations of acetic acid, shown in pic. This
2ml HPLC vial can be replaced by
20ml crimp headspace vialin this experiment.
Conclusion
In short, this is an interesting experiment.
2ml HPLC vial and GC instrument can tell us the quality of whiskey. In our life,
2ml HPLC vial can be widely used. Chromatography is close to our life.
