1. Autosampler Compatibility

Not all autosamplers are alike. Some utilize robotic arms to pick up a sample vial; some use tray rotation while others move the sampling needle to the respective vial coordinates. The dimensions of autosampler vials vary. Most autosamplers are equipped with trays that use 12x32mm vial configurations but some like the Waters Wisp require a 15x45mm configuration. Consult your autosampler’s operating manual or manufacturer to determine the vial dimensions required.

2. Sample Volume

The amount of sample available for analysis is important in choosing the appropriate vial. If only a limited volume of sample is available, you will have to choose between using an insert for your regular autosampler vial, a microvial or a high recovery vial.
Note: Most 12x32 mm autosampler vials hold between 1.5mL to 2.0mL of liquid, depending on where the manufacture measures the vial full. The dimensions for 12x32mm vials are standard between manufacturers but the length of the neck and width/shape of the vial shoulder may vary. 12x32mm microvials have the same outer dimensions but the inside of the vial may be tapered to reduce volume or contain a fused insert. 12x32mm high recovery vials will have an internal conical bottom that allows for maximum sample recovery.

3. Sample Compatibility

The analyte and solvent compatibility should be considered in choosing vials and accessories. For example, amber glass is most often used for light sensitive samples while deactivated glass or plastic vials are used for compounds that are sensitive to glass or stick to the glass itself. Volatile samples require closures that reduce the risk of analyte loss due to evaporation.

4. Vial Composition
Several factors need to be considered when choosing laboratory glass - chemical inertness, durability, light sensitivity, storage conditions, analyte composition and especially exposure to heat/freezing temperatures. Linear Coefficient of Expansion refers to the fractional change in the length of glass per degree of change in temperature, in short, the ability of the glass to tolerate rapid thermal changes. The lower the coefficient of expansion the better the glass can handle temperature change without fracturing.


Classifications for laboratory glass based on its resistance to attack from water were established by the United States Pharmacopoeia, USP.

USP Type 1, Class A, 33 Borosilicate Glass is the most inert and chemically resistant glass widely used in laboratories especially for chromatography applications. Type I glass is composed primarily of silicone and oxygen, with trace amounts of boron and sodium. It has the lowest leaching characteristics and a linear coefficient of expansion of 33.
USP Type 1, Class B, 51 Borosilicate Glass which is composed of silicone and oxygen, trace amounts of boron, sodium and other element is more alkaline than Class A glass but still adequate for laboratory use. All amber borosilicate glass is made of Class B unless otherwise specified and has an expansion coefficient of 51.
Silanized or deactivated glass is borosilicate glass that has undergone further deactivation by treating the surface of the glass with an organosilane. The surface becomes more hydrophobic and inert making the vial suitable for use with pH sensitive compounds, trace analyses and applications requiring long term sample storage.

USP Types II, III and NP glass are manufactured from soda lime which has less chemical resistance than borosilicate.
Polypropylene is a rigid and translucent material, which also comes in a variety of colours and has good chemical resistance for short term storage of most common laboratory chemicals. Resistance decreases over time when using aromatic and halogenated hydrocarbons. Polypropylene vials are often used for ion chromatography because of the extremely low metal content and excellent cleanability with dilute acid followed by deionized water rinse. Polypropylene vials limit the exposure to hazardous materials since they can be incinerated while sealed. Polymethylpentene (TPX) is a rigid transparent material, which has a relatively high melting point with a temperature range of 0°-170°C. TPX vials can be used as alternative to opaque polypropylene vials because they provide maximum clarity. Their chemical resistance is similar to that of polypropylene vials. TPX vials should be used for applications where visual clarity is required or repetitive exposure to higher temperatures such as autoclaving. TPX vials tend to be more brittle at room temperature.

5. Type of Vial Finish and Closures

Autosampler vials are available in a variety of neck finishes and opening diameters. Large mouth or wide ID vials have approximately a 40% wider mouth opening than standard opening vials. The large opening reduces the risk of bent autosampler needles during sampling. Screw thread vials and caps provide low evaporation, reusability, less hand injury during manipulation than crimp seals and require no special tools. All screw threads vials and caps are differentiated by their thread finish as defined by the Glass Packaging Institute, GPI. For screw thread vials, a two part number is assigned. For example, 8-425 neck finish represents a vial with a diameter of 8 mm across the outside of the threads and a thread style of 425.

Screw thread vials and caps are more expensive than crimp seals.
Caps for screw thread vials are available with either an open hole for autosampler use and standard addition or with a solid top for sample storage. One piece polypropylene cap and membrane are also available. These pierceable screw thread caps are designed for one time use and reduce sample preparation time as there is no cap and seal to assemble.

Crimp top vials require lacquered aluminum crimp seals which are relatively inexpensive and, when properly assembled, provide the best seal for long term storage. Crimp seals are not reusuable. A crimping tool is required to seal caps and a decrimper or decapper is required to remove the seals. Several types of hand crimpers are available including the adjustable precision crimpers that Chromatographic Specialties offers. Adjustable hand crimpers feature an adjustable stop on the handle to provide consistent seal tightness every time. Adjusting the hex screw inside the steel crimper jaws will also alter the amount of crimp. Achieving a good crimp is crucial because overcrimping can cause septum coring, bent needles and create a larger hole in the Teflon septum layer than a properly crimped seal. Undercrimping can result in loose seals and sample evaporation. Hand decrimpers quickly and safely remove seals with just a single squeeze of the handle.Decrimpers should be used for applications involving hazardous samples because there is less chance of spillage. Electronic crimpers and decappers are also available. Electronic units reduce hand fatigue, provide consistent crimps and are ideal for laboratories analyzing a lot of samples.

Snap seal vials are less prone to breakage during decapping because more glass is used in the neck of the vial. The snap seal neck finish is compatible with either crimp and/or snap. seals and no special tools are required to remove the cap. These vials are recommended for short term sample storage and non-volatile samples because the seal is not as secure as a crimp or screw thread seal. Shell Vials are an economical alternative to screw thread vials for Water’s HPLC autosamplers or any other autosampler that doesn’t use a robotic arm to move the vials. Most shells vials are sold with a polyethylene cap that has a starburst design for easy needle penetration.